Prothrombin (factor II)

Common pathway · Vitamin K dep. Half-life 60-72 h

Prothrombin is the vitamin K-dependent zymogen that docks on the anionic platelet surface through its Gla domain and is cleaved twice by prothrombinase to release thrombin. Its long half-life of about 60-72 hours is what sets the three to five day delay before warfarin is fully effective.

Traced from the start

  1. Subendothelial collagen and von Willebrand factor
  2. Activated platelet phospholipid surface
  3. Prothrombin (factor II)

Detail

Vitamin K dependent
Yes; the Gla domain binds Ca2+ and phosphatidylserine
Cleaved by
Prothrombinase (Xa-Va-Ca2+-phospholipid) at two arginine bonds, releasing prothrombin fragment 1.2 and thrombin
Half-life
About 60-72 hours, the longest of the vitamin K-dependent factors
Replaced by
Prothrombin complex concentrate, which contains factors II, VII, IX and X with proteins C and S, for warfarin reversal in major bleeding

When it goes wrong

Prothrombin G20210A variant in the 3' untranslated region, which raises the plasma prothrombin level

The second commonest inherited thrombophilia in people of European descent (about 2%), carrying roughly a 2-3 fold venous thromboembolism risk

Acquired hypoprothrombinaemia with a lupus anticoagulant, usually in a child after a viral illness or in SLE

The unusual antiphospholipid patient who bleeds rather than clots: prolonged PT and APTT with a low factor II level

Practise this structure

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