Stratum corneum

Epidermis · Layer 5 (surface) Corneocytes Permeability barrier

Fifteen to twenty layers of anucleate corneocytes embedded in a continuous intercellular lipid matrix. This is the permeability barrier: it limits water loss outwards and drug, allergen and microbe entry inwards.

Detail

Lipid matrix
Roughly 50% ceramides, 25% cholesterol and 10-15% free fatty acids, arranged in lamellae between the corneocytes
Water loss
Restricts insensible loss through skin to about 400-600 mL/day in an adult at rest; loss rises steeply when the layer is destroyed
Desquamation
Kallikrein-family serine proteases digest corneodesmosomes so cells shed individually; the process is balanced against basal mitosis
Drug absorption
The rate-limiting step in percutaneous absorption; thinnest on the eyelid, face, scrotum and flexures, thickest on palms and soles, which is how topical steroid potency is matched to site

When it goes wrong

Loss of the cornified layer over a large area (extensive burn, toxic epidermal necrolysis, erythroderma)

Massive evaporative water loss, hypothermia, hypovolaemia and high-output cardiac failure, plus a wide portal for Staphylococcus and Pseudomonas sepsis

Potent topical corticosteroid applied to the face or under occlusion

Enhanced absorption through thin corneum: skin atrophy, telangiectasia, perioral dermatitis and, over large areas in children, adrenal suppression

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