Bone: osteoblast-osteoclast coupling

Target organ · RANKL up, OPG down Denosumab

Bone holds about 99% of body calcium. PTH does not act on the osteoclast: PTH1R sits on osteoblasts and osteocytes, which answer by raising RANKL and lowering osteoprotegerin, so RANK-bearing osteoclast precursors mature and resorb mineral. Calcitonin acts on the osteoclast directly and switches resorption off.

Traced from the start

  1. Fall in ionised calciumRise in ionised calcium
  2. Calcium-sensing receptor on parathyroid chief cells
  3. Thyroid parafollicular (C) cells
  4. Parathyroid hormoneCalcitonin
  5. Bone: osteoblast-osteoclast coupling

Detail

Signalling
PTH acts on osteoblast and osteocyte PTH1R, raising RANKL and lowering osteoprotegerin, the decoy receptor; RANKL then engages RANK on osteoclast precursors to drive differentiation and resorption
Timing
Rapid calcium efflux from bone fluid within minutes by osteocytic osteolysis, then osteoclastic resorption over hours to days
Calcitonin
Binds the calcitonin receptor on the osteoclast itself and inhibits resorption within minutes
Chemistry
Mineral is hydroxyapatite, Ca10(PO4)6(OH)2, so resorption releases phosphate along with calcium; alkaline phosphatase, an osteoblast product, rises whenever turnover is high

When it goes wrong

Long-standing severe hyperparathyroidism

Osteitis fibrosa cystica: subperiosteal resorption on the radial side of the middle phalanges, a pepper-pot skull, brown tumours and a raised alkaline phosphatase

Denosumab, an anti-RANKL antibody, given to a vitamin D-deficient patient

Severe symptomatic hypocalcaemia; correct vitamin D and calcium before starting, and never stop it without follow-on antiresorptive therapy because of rebound vertebral fractures

Immobilisation, Paget disease of bone or osteolytic metastases

Uncoupled resorption raises serum calcium with an appropriately suppressed PTH

Practise this structure

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